Dr. Dukhande is an assistant professor in Pharmacology in Department of Pharmaceutical Sciences in College of Pharmacy and Health Sciences, St. John’s University. Dukhande is originally from Mumbai, India where he got his B.S. (Pharmacy) from ICT, Mumbai University after which he worked for a year in Unilever Research India where he screened natural actives. He then went on to get PhD from Idaho State University, under the mentorship of Dr. Jim Lai, studying cell death mechanisms . Next, he joined Dr. Matt Gentry’s lab as a postdoc in Biochemistry Department in University of Kentucky, where he studied ubiquitination and phosphorylation in a neurodegenerative epilepsy- Lafora disease. Dr. Dukhande has received several grants and awards including an active NIH grant to study diabetes, an AHA (American Heart Association) postdoctoral fellowship, a research presentation award and travel awards from ASPET (American Society for Pharmacology and Experimental Therapeutics). The unifying theme of Dukhande’s research is to elucidate molecular mechanisms of cell death. His research aims to discover intricate connections between cellular metabolism and cell death. Currently, Dukhande lab is working on research projects to find cure for a rare metabolic epilepsy- Lafora disease and on therapies targeting tumor metabolic reprogramming. He is interested in deciphering the roles of protein post-translational modifications in governing these cellular processes. Dukhande also teaches Pharmacology and Physiology to Pharm.D. students. Our work is supported by a research grant from the National Institute of General Medical Sciences.
Anatomy and Physiology, Pharmacology, Drugs and Infectious Diseases, Active learning
Metabolic disorders (Diabetes, Lafora disease), Cell death, Protein posttranslational modifications, Cancer Pharmacology
PHR 4105 DRUGS & INFECTIOUS DISEASES
PHS 259 CELL SIGNALS AND SYSTEMS
PHS 3504 APPL HUMAN ANATOMY &PHYSIOLOGY
PHS 3509 INTRODUCTION TO PHARMACOLOGY
Barot, S., Abo-Ali, E. M., Zhou, D. L., Palaguachi, C., & Dukhande, V. V. (2019). Inhibition of glycogen catabolism induces intrinsic apoptosis and augments multikinase inhibitors in hepatocellular carcinoma cells. Experimental Cell Research, 381(2), 288–300.
Raththagala, M., Brewer, M. K., Parker, M. W., Sherwood, A. R., Wong, B. K., Hsu, S., Bridges, T. M., Paasch, B. C., Hellman, L. M., Husodo, S., Meekins, D. A., Taylor, A. O., Turner, B. D., Auger, K. D., Dukhande, V. V., Chakravarthy, S., Sanz, P., Woods, Jr, V. L., Li , S., Vander Kooi, C. W., and Gentry, M. S. (2015). Structural mechanism of laforin function in glycogen dephosphorylation and lafora disease.. Molecular cell. vol. 57, pp. 261-72.
Jang, E. R., Shi, P., Bryant, J., Chen, J., Dukhande, V. V., Gentry, M. S., Jang, H., Jeoung, M., and Galperin, E. (2014). HUWE1 is a molecular link controlling RAF-1 activity supported by the Shoc2 scaffold.. Molecular and cellular biology. vol. 34, pp. 3579-93.
Dukhande, V. V., Kawikova, I., Bothwell, A. L., and Lai, J. C. (2013). Neuroprotection against neuroblastoma cell death induced by depletion of mitochondrial glutathione.. Apoptosis : an international journal on programmed cell death. vol. 18, pp. 702-12.
Dukhande, V. V., Sharma, G. C., Lai, J. C., and Farahani, R. (2011). Chronic hypoxia-induced alterations of key enzymes of glucose oxidative metabolism in developing mouse liver are mTOR dependent.. Molecular and cellular biochemistry. vol. 357, pp. 189-97.
Romá-Mateo, C., Solaz-Fuster Mdel, C., Gimeno-Alcañiz, J. V., Dukhande, V. V., Donderis, J., Worby, C. A., Marina, A., Criado, O., Koller, A., De Rodriguez Cordoba, S., Gentry, M. S., and Sanz, P. (2011). Laforin, a dual-specificity phosphatase involved in Lafora disease, is phosphorylated at Ser25 by AMP-activated protein kinase.. The Biochemical journal. vol. 439, pp. 265-75.
Dukhande, V. V., Rogers, D. M., Romá-Mateo, C., Donderis, J., Marina, A., Taylor, A. O., Sanz, P., and Gentry, M. S. (2011). Laforin, a dual specificity phosphatase involved in Lafora disease, is present mainly as monomeric form with full phosphatase activity.. PloS one. vol. 6, pp. e24040.
Lai, J. C., Ananthakrishnan, G., Jandhyam, S., Dukhande, V. V., Bhushan, A., Gokhale, M., Daniels, C. K., and Leung, S. W. (2010). Treatment of human astrocytoma U87 cells with silicon dioxide nanoparticles lowers their survival and alters their expression of mitochondrial and cell signaling proteins.. International journal of nanomedicine. vol. 5, pp. 715-23.
Lai, J. C., Lai, M. B., Jandhyam, S., Dukhande, V. V., Bhushan, A., Daniels, C. K., and Leung, S. W. (2008). Exposure to titanium dioxide and other metallic oxide nanoparticles induces cytotoxicity on human neural cells and fibroblasts.. International journal of nanomedicine. vol. 3, pp. 533-45.
Isaac, A. O., Dukhande, V. V., and Lai, J. C. (2007). Metabolic and antioxidant system alterations in an astrocytoma cell line challenged with mitochondrial DNA deletion.. Neurochemical research. vol. 32, pp. 1906-18.
Dukhande, V. V., Malthankar-Phatak, G. H., Hugus, J. J., Daniels, C. K., and Lai, J. C. (2006). Manganese-induced neurotoxicity is differentially enhanced by glutathione depletion in astrocytoma and neuroblastoma cells.. Neurochemical research. vol. 31, pp. 1349-57.