Maulik R. Patel and Tanaji T. Talele

3D-QSAR Study on 1,3-diarylidene-2-tetralone, 2,6-bis(arylidene)cyclohexanone and 3,5-bis(arylidene)-4-piperidone Series of Cytotoxic Compounds

Maulik R. Patel, Department of Pharmaceutical Sciences, College of Pharmacy and Allied Health Professions
Tanaji T. Talele, Department of Pharmaceutical Sciences, College of Pharmacy and Allied Health Professions
Jonathan R. Dimmock, College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, Sask., Canada

Abstract
In a continuing effort to develop more potent cytotoxic compounds in our laboratory, we developed 3D-QSAR models for 1,3-diarylidene-2-tetralone, 2,6-bis(arylidene)cyclohexanone and 3,5-bis(arylidene)-4-piperidone analogues previously shown to exhibit cytotoxic activity in L1210, Molt4 C/8, and CEM cells (Dimmock et al., J. Med. Chem. 2001, 44, 586–593; Eur. J. Med. Chem. 2000, 35, 967-977; Eur. J. Med. Chem. 2002, 37, 813-824; Eur. J. Med. Chem. 2003, 38, 169-177).  The CoMFA models with steric and electrostatic fields provided satisfactory statistical data (r2cv 0.485, r2ncv 0.834, r2pred 0.591), (r2cv 0.532, r2ncv 0.850, r2pred 0.729), and (r2cv 0.561, r2ncv 0.864, r2pred 0.666) for cytotoxicity in L1210, Molt4 C/8, and CEM cells, respectively.  The CoMSIA models with steric, electrostatic, hydrophobic, and H-bond donor fields exhibited r2cv 0.513, r2ncv 0.833, and r2pred 0.562 for cytotoxicity in L1210 cells while best CoMSIA model was obtained by combination of steric, electrostatic, and hydrophobic fields yielding statistically significant data (r2cv 0.531, r2ncv 0.828, r2pred 0.652) and (r2cv 0.560, r2ncv 0.841, r2pred 0.729) for cytotoxicity in Molt4 C/8 and CEM cells, respectively.  The information obtained from CoMFA and CoMSIA 3D contour maps can be used in the design of more potent compounds as cancer chemotherapeutics.